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COX7A2L Antibody

46542
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  Catalog No. Package Size Price
46542 100ul $279.00

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Description
  • Product Name COX7A2L Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antigen affinity purification
  • Applications WB IHC
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total COX7A2L protein.
  • Immunogen Type peptide
  • Immunogen Description Full length fusion protein of human COX7A2L
  • Target Name COX7A2L
  • Alternative Names EB1; SIG81; COX7AR; COX7RP
  • Accession No. Swiss-Prot:O14548
    NCBI Gene ID:9167
    NCBI Protein:BC007095
  • Calculated MW 13 kDa
  • Concentration 3.3mg/ml
  • Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:200-1:1000
Immunohistochemistry: 1: 30-150

Images
  • COX7A2L Antibody - SAB | Signalway Antibody

    Gel: 12%SDS-PAGE
    £¬ Lysate: 40 ¦Ìg£¬ Lane 1-2:Mouse brain tissue and Mouse adrenal gland tissue lysates£¬
    Primary antibody: 46542(COX7A2L Antibody) at dilution 1/500£¬
    Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution£¬
    Exposure time: 40 seconds

  • COX7A2L Antibody - SAB | Signalway Antibody

    The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46542(COX7A2L Antibody) at dilution 1/30. (Original magnification: x200)

  • COX7A2L Antibody - SAB | Signalway Antibody

    The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 46542(COX7A2L Antibody) at dilution 1/30. (Original magnification: x200)

Background
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen. Several transcript variants, some protein-coding and others non-protein coding, have been found for this gene.
    Please let us know if you have published research using #46542 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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