• Product NameHIPK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HIPK2 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human HIPK2
• Target NameHIPK2
• Alternative Names    PRO0593
• Accession No. Swiss-Prot:Q9H2X6
  NCBI Gene ID:28996
  NCBI Protein:NP_001106710
• UniprotQ9H2X6
• Gene ID28996;
• Calculated MW 131 kDa
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1: 30-150

This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  lysate: 40 ¦Ìg, Lane: 231 cell lysate,
  Primary antibody: 46574£¨HIPK2 Antibody) at dilution 1/850
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 2 minutes
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 46574(HIPK2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46574(HIPK2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting