• Product NamePGK2 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human PGK2 (NP_620061.2).
• Alternative Names    PGK2;HEL-S-272;PGKB;PGKPS;dJ417L20.2
• Accession No. Swiss Prot:P07205
  GeneID:5232
• UniprotP07205
• Calculated MW 44kDa
• SDS-PAGE MW 45kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.

• Western blot analysis of extracts of various cell lines, using PGK2 at 1:3000 dilution.
• Immunofluorescence analysis of C6 cells using PGK2 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of L929 cells using PGK2 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human mammary cancer using PGK2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat testis using PGK2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse testis using PGK2 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting