• Product NameCD34 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human CD34 (NP_001020280.1).
• Alternative Names    CD34;CD34 molecule;GIG3;MORT1
• Accession No. Swiss Prot:P28906
  GeneID:947
• UniprotP28906
• Calculated MW 35kDa/40kDa
• SDS-PAGE MW 110kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.

• Immunohistochemistry of paraffin-embedded human placenta using CD34 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using CD34 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat kidney using CD34 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of rat brain using CD34 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of human placenta using CD34 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using CD34 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting