• Product NameGLS Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human Glutaminase (Glutaminase (GLS)) (NP_055720.3).
• Alternative Names    GLS;AAD20;GAC;GAM;GLS1;KGA
• Accession No. Uniprot:O94925
  GeneID:2744
• UniprotO94925
• Gene ID2744
• Calculated MW 17kDa/65kDa/73kDa
• SDS-PAGE MW 65-73KDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

• Western blot analysis of extracts of various cell lines, using Glutaminase (Glutaminase (GLS)) antibody.
• Immunofluorescence analysis of NIH/3T3 cells using Glutaminase (GLS) antibody.
• Immunofluorescence analysis of PC-12 cells using Glutaminase (GLS) antibody.
• Immunofluorescence analysis of SH-SY5Y cells using Glutaminase (GLS) antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting