• Product NameTRAP1 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human TRAP1 (NP_057376.2).
• Alternative Names    TRAP1;HSP 75;HSP75;HSP90L;TRAP-1
• Accession No. Swiss Prot:Q12931
  GeneID:10131
• UniprotQ12931
• Calculated MW 74kDa/80kDa
• SDS-PAGE MW 80kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.

• Immunohistochemistry of paraffin-embedded Rat brain using TRAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded Human colon carcinoma using TRAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded Mouse kidney using TRAP1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of NIH/3T3 cells using TRAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U2OS cells using TRAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using TRAP1 at 1:3000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting