• Product NameCLIC4 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human CLIC4 (NP_039234.1).
• Alternative Names    CLIC4;CLIC4L;H1;MTCLIC;huH1;p64H1
• Accession No. Swiss Prot:Q9Y696
  GeneID:25932
• UniprotQ9Y696
• Calculated MW 28kDa
• SDS-PAGE MW 29kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:100

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

• Western blot analysis of extracts of various cell lines, using CLIC4 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat brain using CLIC4 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of A-549 cells using CLIC4 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting