• Product NameNAT10 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Rat
• Immunogen DescriptionRecombinant fusion protein of human NAT10 (NP_078938.2).
• Alternative Names    NAT10;ALP;NET43;Kre33
• Accession No. Swiss Prot:Q9H0A0
  GeneID:55226
• UniprotQ9H0A0
• Calculated MW 107kDa/115kDa
• SDS-PAGE MW 116kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization.

• Western blot analysis of extracts of various cell lines, using NAT10 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
• Immunofluorescence analysis of MCF-7 cells using NAT10 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting