• Product NameNBN Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human NBN
• Alternative Names    NBN;AT-V1;AT-V2;ATV;NBS;NBS1;P95;nibrin
• Accession No. Swiss Prot:O60934
  GeneID:4683
• UniprotO60934
• Calculated MW 84kDa
• SDS-PAGE MW 100kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

• Immunofluorescence analysis of U2OS cells using NBN at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using NBN at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting