• Product NameACAT2 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human ACAT2 (NP_005882.2).
• Alternative Names    ACAT2
• Accession No. Swiss Prot:Q9BWD1
  GeneID:39
• UniprotQ9BWD1
• Calculated MW 41kDa/44kDa
• SDS-PAGE MW 41kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using ACAT2 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat ovary using ACAT2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon carcinoma using ACAT2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human oophoroma using ACAT2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using ACAT2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat brain using ACAT2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human oophoroma using ACAT2 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human breast cancer using ACAT2 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of L929 cells using ACAT2 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting