• Product NameRNF5 Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human RNF5 (NP_008844.1).
• Alternative Names    RNF5;RING5;RMA1
• Accession No. Swiss Prot:Q99942
  GeneID:6048
• UniprotQ99942
• Calculated MW 19kDa
• SDS-PAGE MW 21kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:100

The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.

• Western blot analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat liver using RNF5 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human liver cancer using RNF5 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using RNF5 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting