• Product NameNEFH Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human Neurofilament H (NP_066554.2).
• Alternative Names    CMT2CC;NFH;NEFH
• Accession No. Uniprot:P12036
  GeneID:4744
• UniprotP12036
• Gene ID4744
• Calculated MW 105kDa/112kDa
• SDS-PAGE MW 150KDa/220KDa
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

• Western blot analysis of extracts of various cell lines, using Neurofilament H antibody.
• Immunofluorescence analysis of mouse brain using Neurofilament H Rabbit pAb.
• Immunofluorescence analysis of C6 cells using Neurofilament H Rabbit pAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting