• Product NameCACYBP Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human CACYBP (NP_055227.1).
• Alternative Names    CACYBP;GIG5;PNAS-107;S100A6BP;SIP
• Accession No. Swiss Prot:Q9HB71
  GeneID:27101
• UniprotQ9HB71
• Calculated MW 8kDa/21kDa/26kDa
• SDS-PAGE MW 26kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:1000 - 1:2000
  IF 1:50 - 1:200

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using CACYBP at 1:1000 dilution.
• Immunofluorescence analysis of NIH-3T3 cells using CACYBP at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of HeLa cells using CACYBP at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting