• Product Namep300 (phospho Ser89) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-p300 (S89) Polyclonal Antibody detects endogenous levels of p300 protein only when phosphorylated at S89.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human p300 around the phosphorylation site of Ser89. AA range:55-104
• Alternative Names    EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300
• Accession No. Swiss Prot:Q09472
  GeneID:2033
• UniprotQ09472
• Gene ID2033
• Calculated MW 264kd
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• 
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/5000. Not yet tested in other applications.

E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting