• Product Namep130 Cas (phospho Tyr249) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-p130 Cas (Y249) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y249.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr249. AA range:215-264
• Alternative Names    BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
• Accession No. Swiss Prot:P56945
  GeneID:9564
• UniprotP56945
• Gene ID9564
• SDS-PAGE MW 130
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/10000. Not yet tested in other applications.

BCAR1, Cas family scaffolding protein(BCAR1) Homo sapiens BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting