• Product NameAnti-CD22 antibody(DM12),Rabbit mAb
• Host SpeciesRabbit
• Clone No.DM12
• IsotypeRabbit IgG
• PurificationPurified from cell culture supernatant by affinity chromatography
• ApplicationsELISA, Flow Cyt, WB, IF
• Species ReactivityHuman
• Immunogen DescriptionRecombinant human CD22 (Asp20-Arg687) produced by using human HEK293 cells
• Alternative Names    SIGLEC-2, SIGLEC2
• Calculated MW 95kDa
• Formulation Preservative: 0.1% Procline 300 Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
• Storage Store at -20˚C for 13 months (Avoid repeated freezing and thawing)

• Flow Cyt 1/100

• Format: Liquid

CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC1.

• Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti-CD22 monoclonal antibody 15μg/ml (clone: DM12) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
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• Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM12) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
• Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
• Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting