• Product NameAnti-CD123 antibody(DM33), Rabbit mAb
• Host SpeciesRabbit
• Clone No.DM33
• IsotypeRabbit IgG
• PurificationPurified from cell culture supernatant by affinity chromatography
• ApplicationsELISA,Flow Cyt
• Species ReactivityInterleukin 3 receptor alpha
• Immunogen DescriptionRecombinant human? CD123(Thr19-Arg305) (PME100003) produced by using human HEK293 cells
• Alternative Names    IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, MGC34174, IL-3 R alpha
• Calculated MW 39kDa
• Formulation Preservative: 0.1% Procline 300 Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
• Storage Store at -20˚C for 12 months (Avoid repeated freezing and thawing)

• ELISA 1/5000-10000;Flow Cyt 1/100

• Format: Liquid

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-??

• Figure 1. Expi 293 cell line transfected with irrelevant protein (left) and human CD123(right) were surface stained with Rabbit anti-CD123 monoclonal antibody 1μg/ml (clone: DM33) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
• 
• Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD123 monoclonal antibody ( clone: DM33 ) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
• Figure 3. Affinity ranking of different Rabbit anti-CD123 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting