• Product NameCDC2(Phospho-Tyr15) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from Human CDC2.
• Target NameCDC2
• ModificationPhospho
• Alternative Names    CDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
• Accession No. Swiss-Prot: P06493
  NCBI Protein: NP_001163877.1
• UniprotP06493
• Gene ID983;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 34kd
  Western blotting: 1:500~1:1000

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Y Gu, et al. (1992) EMBO J. 11(11): 3995

• Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) Antibody #11244.
• Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #11244.
• Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #11244.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting