- Product NameRecombinant Human Leukemia inhibitory factor protein(LIF) (Active)
- Brief DescriptionRecombinant Protein
- Purification>98% as determined by SDS-PAGE and HPLC.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng,ml, corresponding to a specific activity of 1.0 107 IU,mg.
- Immunogen DescriptionExpression Region:23-202aa
Sequence Info:Full Length of Mature Protein - Alternative Names LIF, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin
- Accession No. P15018
- UniprotP15018
- Gene ID3976;
- Calculated MW 19.7 kDa
- Tag Info NO-tagged
- Target Sequence SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
- Formulation 0.2 μm filtered PBS, pH 7.4 ,lyophilized
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Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
