- Product NameRecombinant Human SPARC protein(SPARC) (Active)
- Brief DescriptionRecombinant Protein
- Purification>95% as determined by SDS-PAGE and HPLC.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ?g,mL, corresponding to a specific activity of > 333 IU,mg.
- Immunogen DescriptionExpression Region:18-303aa
Sequence Info:Full Length of Mature Protein - Alternative Names Basement-membrane protein 40, Osteonectin, ON, Secreted protein acidic and rich in cysteine,
- Accession No. P09486
- UniprotP09486
- Gene ID6678;
- Calculated MW 36.1 kDa
- Tag Info N-terminal 6xHis-tagged
- Target Sequence MSYYHHHHHH DYDIPTTENL YFQGAMGSA+P QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI
- Formulation 0.2 ?m filtered PBS, pH 7.4 ,lyophilized
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Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
