• Product NameConnexin 43 (phospho-Ser368) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Connexin43 only when phosphorylated at serine 368.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 368 (R-A-S(p)-S-R) derived from Human Connexin 43.
• Target NameConnexin 43
• ModificationPhospho
• Alternative Names    CX43; CXA1; CXN-43; GJA1;
• Accession No. Swiss-Prot: P17302
  NCBI Protein: NP_000156.1
• UniprotP17302
• Gene ID2697;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
   

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211
Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353,
Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654
W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444
 

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 (Phospho-Ser368) Antibody #11258 (left) or the same antibody preincubated with blocking peptide (right).
• Western blot analysis of extracts from 293 cells untreated or treated with PMA using Connexin 43 (phospho-Ser368) Antibody #11258.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting