• Product NameTRIM25 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB ICC
• Species ReactivityHu Ms
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 19 amino acid peptide from near the carboxy terminus of human TRIM25.
• Target NameTRIM25
• Alternative Names    Tripartite motif-containing 25, estrogen responsive finger protein, EFP
• Accession No. Swiss-Prot:Q14258
  Gene ID:7706
• UniprotQ14258
• Gene ID7706;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

• Western blot analysis of TRIM25 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/mL.
• Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting