• Product NameCREB(Phospho-Ser129) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of CREB only when phosphorylated at serine 129.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 129 (I-L-S(p)-R-R) derived from Human CREB.
• Target NameCREB
• ModificationPhospho
• Alternative Names    CREB-1; CREB1;
• Accession No. Swiss-Prot: P16220
  NCBI Protein: NP _004370.1
• UniprotP16220
• Gene ID1385;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948.
Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543.
Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.
Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.

• Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with UV(lane 2) using CREB(Phospho-Ser129) Antibody #11273.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser129) Antibody #11273(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting