• Product NameMED4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• SpecificityAt least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 17 amino acid peptide from near the carboxy terminus of human MED4.
• Target NameMED4
• Alternative Names    Mediator of RNA polymerase II transcription subunit 4, vitamin d receptor interacting protein, vdrip, drip36, trap36
• Accession No. Swiss-Prot:Q9NPJ6
  Gene ID:29079
• UniprotQ9NPJ6
• Gene ID29079;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.

• Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 ug/mL.
• Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting