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MEK1(Phospho-Thr291) Antibody #11294

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Datasheet PDF
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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
Availability:Yes
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Description
  • Product NameMEK1(Phospho-Thr291) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • ApplicationsWB
  • Species ReactivityHu Ms Rt
  • SpecificityThe antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 291 (P-R-T(p)-P-G derived from Human MEK1.
  • Target NameMEK1
  • ModificationPhospho
  • Alternative Names    ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
  • Uniprot Q02750
  • Gene ID 5604;
  • Accession No. Swiss-Prot: Q02750
    NCBI Protein: NP_002746.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Background

Published Papers

Magnesium deprivation inhibits a MEK�CERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells. In Life Sci on 2010 May 8 by Akira Ikari, Kosuke Atomi, et al..PMID: 20338184 , , (2010),
PMID: 20338184
CD40-expressing Plasmid Induces anti-CD40 Antibody and Enhances Immune Responses to DNA Vaccination.In J Gene Med on 2010 Jan by Hanqian Xu, Gan Zhao,et al..PMID:19950201, , (2010),
PMID: 19950201
Please let us know if you have published research using #11294 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep