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GAPDH Antibody

24830
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  Catalog No. Package Size Price
24830 100ul $390.00

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Description
  • Product Name GAPDH Antibody
  • Host Species Chicken
  • Clonality Polyclonal
  • Purification Affinity chromatography purified via peptide column
  • Applications ELISA WB
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide
  • Immunogen Description Raised against a 16 amino acid peptide from near the carboxy terminus of human GAPDH.
  • Target Name GAPDH
  • Alternative Names Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
  • Accession No. P04406
  • Concentration 1mg/ml
  • Formulation Supplied in PBS containing 0.02% sodium azide.
  • Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Images
  • GAPDH Antibody - SAB | Signalway Antibody

    Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at 1 ug/mL.

Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
    Please let us know if you have published research using #24830 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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