• Product NameAPAF1 Antibody FITC Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsFlow-Cyt IF
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic peptide aa 3-44 1248 derived from human Apaf-1
• ConjugatesFITC
• Excitation Emission494nm 518nm
• Target NameAPAF1NT
• Alternative Names    CED4; APAF-1; Apoptotic protease-activating factor 1; APAF1; KIAA0413
• Accession No. Swiss-Prot#O14727
  NCBI Gene ID317
• UniprotO14727
• Gene ID317;
• Cell Localization Cytoplasm
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• Flow-Cyt=1:50-200 IF=1:50-200

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting