• Product NameCDKN2A Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-8C9
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse
• Immunogen DescriptionRecombinant protein of human CDKN2A/p16INK4a
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
• Accession No. Swiss-Prot:P42771
  GeneID:1029
• UniprotP42771
• Gene ID1029
• Calculated MW Calculated MW: 17 kDa; Observed MW: 17 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; ICC/IF: 1/20-1/100;

Swiss-Prot Acc.P42771.Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

• Western blot detection of CDKN2A/p16INK4a in 293 cell lysates using CDKN2A/p16INK4a Rabbit mAb(1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
• Western blot detection of CDKN2A-p16INK4a in 3T3,Hela cell lysates using CDKN2A/p16INK4a Rabbit mAb(1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
• Immunofluorescence of CDKN2A/p16INK4a(green) in Hela cells using CDKN2A/p16INK4a Rabbit mAb at dilution 1/5, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting