• Product NameNCK2 Rabbit mAb
• Clone No.S01-3C6
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;ICC/IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Nck beta
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    GRB4; NCKbeta
• Accession No. Swiss-Prot:O43639
  GeneID:8440
• UniprotO43639
• Gene ID8440
• Calculated MW Calculated MW: 43 kDa; Observed MW: 47 kDa
• Concentration 1.5mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:2000;
  IHC 1:100-1:200;
  ICC/IF 1:50-1:200

Swiss-Prot Acc.O43639.Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

• Immunofluorescence of Nck beta(green) in Hela cells using Nck beta Rabbit mAb at dilution 1/200, and DAPI(blue)
• Western blot analysis of Nck2 expression in SH-SY5Y cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting