• Product NameAKT1 2 3 (Phospho-Ser472 Ser473 Ser474) Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB IHC-P IHC-F
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide aa 450-480 480 derived from human AKT1 around the phosphorylation site of Ser473
• ConjugatesHRP
• Excitation EmissionN A
• Target NameAKT1 2 3 (Ser472 Ser473 Ser474)
• Alternative Names    Akt; RAC-alpha serine threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
• Accession No. Swiss-Prot#P47196
  NCBI Gene ID24185
• UniprotP47196
• Gene ID24185;
• Cell Localization Cytoplasm, Nucleus
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting