• Product NameRecombinant Human Macrophage Inflammatory Protein-5/CCL15
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
• EndotoxinLess than 1 EU/ug of rHuMIP-5/CCL15 as determined by LAL method.
• Alternative Names    C-C Motif Chemokine 15, LKN-1, Mrp-2b, NCC-3, Small-inducible Cytokine A15
• UniprotQ16663
• Gene ID6359
• Calculated MW Approximately 10.2 kDa, a single non-glycosylated polypeptide chain containing 92 amino acids.
• Target Sequence QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI
• Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ��C as supplied.- 1 month, 2 to 8 ��C under sterile conditions after reconstitution.- 3 months, -20 to -70 ��C under sterile conditions after reconstitution.

Human CCL15 is belonging to the CC chemokine family and shares 35 % amino acid homology with human HCC1 (CCL14). CCL15 is most abundant in heart, skeletal muscle and adrenal gland, and low expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. CCL15 has several cleaved chains. All of them are more potent chemoattractants than CCL15.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting