• Product NamePKC alpha Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityPKC alpha Antibody detects endogenous levels of total PKC alpha
• Immunogen DescriptionA synthesized peptide derived from human PKC alpha
• Alternative Names    EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;
• Accession No. Uniprot:P17252
• UniprotP17252
• Calculated MW 75kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

• PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.

PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.

• Western blot analysis of PKC alpha expression in HEK293 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody.
• Immunofluorescent analysis of Hela cells, using PKC alpha Antibody .
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting