• Product Nameactive + pro Caspase 3 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC
• Species ReactivityHuman;Mouse;Rat
• Specificityactive + pro Caspase 3 Antibody detects endogenous levels of total active + pro Caspase 3
• Immunogen DescriptionA synthesized peptide derived from human active + pro Caspase 3
• Alternative Names    Apopain precursor; CASP-3; CPP-32; CPP32; Caspase-3; Cysteine protease CPP32; ICE3; SCA-1; SREBP cleavage activity 1;
• Accession No. Uniprot:P42574
• UniprotP42574
• Calculated MW 17,32 kDa
• SDS-PAGE MW 17, 32 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50

• This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

• All lanes : Cleaved-Caspase 3 p17 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Hela whole cell lysates
  Lane 2 : JK whole cell lysates
  Lane 3 : 293T whole cell lysates
  Lane 4 : HUVEC whole cell lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 17,32 kDa
  Observed band size: 17,32 kDa
  
  Exposure time: 5 seconds
• All lanes : Cleaved-Caspase 3 p17 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Mouse Liver lysates
  Lane 2 : Mouse Spleen lysates
  Lane 3 : Rat Liver lysates
  Lane 4 : Rat Spleen lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 17,32 kDa
  Observed band size: 17,32 kDa
  
  Exposure time: 5 seconds
• All lanes : Cleaved-Caspase 3 p17 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Wild-type Hela cell lysate
  Lane 2 : Cleaved-Caspase 3 p17 knockdown Hela cell lysate
  
  Lysates/proteins at 20 µg per lane.
• Formalin-fixed, paraffin-embedded human pituitary tumor tissue stained for Cleaved-Caspase 3 p17 using 58822 at 1/100 dilution in immunohistochemical analysis.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting