• Product NameNG2 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;IF
• Species ReactivityHuman
• SpecificityNG2 Antibody detects endogenous levels of total NG2
• Immunogen DescriptionA synthesized peptide derived from human NG2
• Alternative Names    NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG;
• Accession No. Uniprot:Q6UVK1
• UniprotQ6UVK1
• Calculated MW 251 kDa
• SDS-PAGE MW 251 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 FC 1:50

• Play a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin.

Play a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin.

• All lanes : CSPG4 Rabbit mAb at 1/1k dilution
  
  Lane 1 : A172 whole cell lysates
  Lane 2 : A375 whole cell lysates
  Lane 3 : Hela whole cell lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 251 kDa
  Observed band size: 251 kDa
  
  Exposure time: 20 seconds
• All lanes : CSPG4 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Wild-type Hela cell lysate
  Lane 2 : CSPG4 knockdown Hela cell lysate
  
  Lysates/proteins at 20 µg per lane.
• Formalin-fixed, paraffin-embedded human colon carcinom tissue stained for CSPG4 using 59290 at 1/100 dilution in immunohistochemical analysis.
• Immunocytochemistry/ Immunofluorescence CSPG4 antibody (59290)
  
  59290 staining CSPG4 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
  Samples were incubated with 59290 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
  The negative control is shown in bottom right hand panel - for the negative control, purified 59290 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
  Nuclei were counterstained with DAPI.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting