• Product NameEg5 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF IP
• Species ReactivityHuman
• SpecificityEg5 Antibody detects endogenous levels of total Eg5
• Immunogen DescriptionA synthesized peptide derived from human Eg5
• Alternative Names    EG5; HKSP; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein KIF11; KNSL1; MCLMR; TR-interacting protein 5; TRIP5;
• Accession No. Uniprot:P52732
• UniprotP52732
• Calculated MW 119kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50

• Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

• Western blot analysis of Eg5 expression in Raji cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting