• Product NameYes1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;IF
• Species ReactivityHuman;Mouse;Rat
• SpecificityYes1 Antibody detects endogenous levels of total Yes1
• Immunogen DescriptionA synthesized peptide derived from human Yes1
• Alternative Names    c Yes; HsT441; P61 YES;Yes; YES1;
• Accession No. Uniprot:P07947
• UniprotP07947
• Calculated MW 61 kDa
• SDS-PAGE MW 61 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

• Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.

Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.

• All lanes : YES1 Rabbit mAb at 1/1k dilution
  
  Lane 1 : JK whole cell lysates
  Lane 2 : HUVEC whole cell lysates
  Lane 3 : K562 whole cell lysates
  Lane 4 : HepG2 whole cell lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 61 kDa
  Observed band size: 61 kDa
  
  Exposure time: 7 seconds
• All lanes : YES1 Rabbit mAb at 1/1k dilution
  
  Lane 1 : 3T3 whole cell lysates
  Lane 2 : PC12 whole cell lysates
  Lane 3 : C2C12 whole cell lysates
  Lane 4 : C6 whole cell lysates
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  
  Predicted band size: 61 kDa
  Observed band size: 61 kDa
  
  Exposure time: 30 seconds
• All lanes : YES1 Rabbit mAb at 1/1k dilution
  
  Lane 1 : Wild-type Hela cell lysate
  Lane 2 : YES1 knockdown Hela cell lysate
  
  Lysates/proteins at 20 µg per lane.
• Formalin-fixed, paraffin-embedded mouse lung tissue stained for YES1 using 59790 at 1/100 dilution in immunohistochemical analysis.
• Formalin-fixed, paraffin-embedded rat kidney tissue stained for YES1 using 59790 at 1/100 dilution in immunohistochemical analysis.
• Immunocytochemistry/ Immunofluorescence YES1 antibody (59790)
  
  59790 staining YES1 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
  Samples were incubated with 59790 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
  The negative control is shown in bottom right hand panel - for the negative control, purified 59790 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
  Nuclei were counterstained with DAPI.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting