• Product NameNemo-like kinase (Phospho-Thr298) Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB,,IHC-P,IHC-F,ICC
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298
• ConjugatesHRP
• Excitation EmissionN A
• Target NameNemo-like kinase Thr298
• Alternative Names    Nemo-like kinase Thr298; Nemo-like kinase T298; p-Nemo-like kinase Thr298; p-Nemo-like kinase T298; DKFZp761G1211; FLJ21033; LAK1; Nemo like kinase; Nemo-like kinase; Nlk; NLK_HUMAN; Protein LAK1; Serine threonine protein kinase NLK; Serine threonine-protein kinase NLK.
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200

Nemo-like kinase (NLK) plays a role in cell fate determination and is required for differentiation of bone marrow stromal cells. It acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. It may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. (referenced from swissprot)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting