• Product NameNFAT1(Phospho-Ser53) Antibody
• Brief DescriptionRabbit Polyclonal
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• ApplicationsWB
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of NFAT1 only when phosphorylated at serine 53.
• Immunogen TypePeptide
• Immunogen DescriptionA synthesized peptide derived from human NFAT1 around the phosphorylation site of Ser53.
• ConjugatesUnconjugated
• Target NameNFATC2
• UniprotQ13469
• Gene ID4773
• Calculated MW 100kDa
• Concentration 1mg/ml
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB 1:500-1:2000

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed:15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed:21871017). Is involved in the negative regulation of chondrogenesis (PubMed:35789258). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca2+ signaling microdomains where store-operated Ca2+ influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.

• All lanes : NFAT1(Phospho-Ser53) Antibody at 1/1K dilution
  Lane 1 : treated with blocking peptide
  Lane 2 : Hela cells(heat shock treatment) lysates
  Lysates/proteins at 20 ug per lane.
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
  Predicted band size: 100 kDa
  Observed band size: 100-150 kDa
  
  Exposure time: 4 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting