• Product NamePCNA antibody PE Conjugated
• Brief DescriptionRabbit Polyclonal
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity purified by Protein A.
• ApplicationsFC, IF
• Species ReactivityHuman Rat
• Immunogen TypePeptide
• Immunogen Descriptionpeptide derived from human PCNA
• ConjugatesPE
• Target NamePCNA
• Calculated MW 29kDa
• Concentration 1mg/ml
• Formulation Liquid in 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Store at +4˚C for short term. Store at -20˚C for long term. Avoid freeze/thaw cycle.

• FC 1:50-200, IF 1:50-200

Proliferation Marker Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure��

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting