• Product NameFbpB antibody
• Brief DescriptionRabbit Polyclonal
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity purified by Protein A.
• ApplicationsWB
• Immunogen TypePeptide
• Immunogen Descriptionpeptide derived from Mycobacterium tuberculosis Ag85B: 231-325/325
• Target NameMycobacterium tuberculosis Ag85B
• Calculated MW 31 kDa
• Concentration 1mg/ml
• Formulation Liquid in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
• Storage Store at +4˚C for short term. Store at -20˚C for long term. Avoid freeze/thaw cycle.

• WB 1:500-2000

Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall.

• All lanes : FbpB antibody at 1/1k dilution
  Lane 1: Recombinant M.tuberculosis fbpB protein
  Lysates/proteins at 30 ug per lane.
  Secondary
  All lanes : IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa
  Observed band size: 31 kDa
  Exposure time: 7 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting