CD127 Rabbit mAb - SAB | Signalway Antibody

Catalog No.	Package Size	Price	Quantity
62251-1	100ul	$599.00

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Description

Product NameCD127 Rabbit mAb
Host SpeciesRabbit
ClonalityMonoclonal
Clone No.SR4557
PurificationProtein A affinity purified.
ApplicationsWB;IF;IHC;FC
Species ReactivityHuman;Mouse
Immunogen TypeProtein
Immunogen DescriptionRecombinant protein within Human CD127
Conjugatesunconjugated
Target NameCD127
UniprotP16871
Calculated MW 52 kDa
Concentration 1ug/ul
Formulation 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Application Details

Images

Western blot analysis of CD127 on K562 cell lysates with CD127 at 1/500 dilution.
Lysates/proteins at 10 ug/Lane.
Predicted band size: 52 kDa
Observed band size: 70 kDa
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with CD127 at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of SiHa cells labeling CD127 at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with CD127 at 1/50 dilution in 2% negative goat serum overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Flow cytometric analysis of K-562 cells labeling CD127.
Cells were fixed and permeabilized. Then stained with the primary antibody ( 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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CD127 Rabbit mAb #62251

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