CD33 Rabbit mAb - SAB | Signalway Antibody

Catalog No.	Package Size	Price	Quantity
62255-1	100ul	$599.00

Datasheet Images Protocol

Description

Product NameCD33 Rabbit mAb
Host SpeciesRabbit
ClonalityMonoclonal
Clone No.SR4561
PurificationProtein A affinity purified.
ApplicationsWB;IF;FC
Species ReactivityHuman
Immunogen TypeProtein
Immunogen DescriptionRecombinant protein within human CD33
Conjugatesunconjugated
Target NameCD33
UniprotP20138
Calculated MW 40 kDa
Concentration 1ug/ul
Formulation PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Application Details

Images

Western blot analysis of CD33 on different lysates with CD33 at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: TF-1 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Jurkat cell lysate (negative)
Lane 5: SK-MEL-28 cell lysate (negative)
Lysates/proteins at 20 ug/Lane.
Predicted band size: 40 kDa
Observed band size: 70 kDa
Western blot analysis of CD33 on different lysates with CD33 at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 cell lysate treated with deglycosylation
Predicted band size: 40 kDa
Observed band size: 40/70 kDa (Glycosylated)
Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling CD33 at 1/200 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with CD33 at 1/200 dilution in 1% BSA in PBST overnight at 4℃. Goat Anti-Rabbit IgG H&L (iFluor 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Flow cytometric analysis of TF-1 cells labeling CD33.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1ug/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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CD33 Rabbit mAb #62255

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