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Recombinant Human Tumor Necrosis Factor-a (rHu TNF-a) #70301

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Catalog No. Package Size Price Quantity
70301 50ul $235.00
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Description
  • Product NameRecombinant Human Tumor Necrosis Factor-a (rHu TNF-a)
  • Brief DescriptionRecombinant Protein
  • Host SpeciesE.coli
  • Purification> 98 % by SDS-PAGE and HPLC analyses.
  • Species ReactivityHu
  • Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng ml, corresponding to a specific activity of > 2.0 ⅹ 107 IU mg in the presence of actinomycin D.
  • EndotoxinLess than 1.0 EU/ng of rHuTNF-a/TNFSF2 as determined by LAL method.
  • Target NamerHu TNF-a
  • Alternative Names    Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin
  • Accession No. accession:P01375
    GeneID:7124
  • UniprotP01375
  • Gene ID7124;
  • Calculated MW Approximately 17.5 kDa, a sing
  • SDS-PAGE MW Sterile Filtered White lyophil
  • Target Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
  • Formulation Lyophilized from a 0.2 ng m filtered concentrated solution in 20 mM PB, 10 mM Nacl, pH 7.0.
  • Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
  • Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
Background
Regerences

Published Papers

Xanthatin Induces Cell Cycle Arrest at G2/M Checkpoint and Apoptosis via Disrupting NF-kB Pathway in A549 Non-Small-Cell Lung Cancer Cells.In Molecules on 2012 Mar 26 by Lei Zhang, Junshan Ruan,et al..PMID:22450683, , (2012),
PMID: 22450683
Please let us know if you have published research using #70301 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep