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Recombinant Human Fibroblast Growth Factor-basic (rHu bFGF ) #70402

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Catalog No. Package Size Price Quantity
70402 100ug $359.00
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Description
  • Product NameRecombinant Human Fibroblast Growth Factor-basic (rHu bFGF )
  • Brief DescriptionRecombinant Protein
  • Host SpeciesE.coli
  • Purification> 96 % by SDS-PAGE and HPLC analyses.
  • Species ReactivityHu
  • Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb c 3T3 cells is less than 0.05 ng ml, corresponding to a specific activity of > 2.0 ⅹ 107 IU mg.
  • EndotoxinLess than 1 EU/μg of rHubFGF as determined by LAL method.
  • Target NamerHu bFGF
  • Alternative Names    FGF-2, HBGF-2
  • Uniprot P09038
  • Gene ID 2247;
  • Accession No. accession:P09038
    GeneID:2247
  • Calculated MW Approximately 16.5 kDa, a sing
  • SDS-PAGE MW Sterile Filtered White lyophil
  • Target Sequence MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
  • Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
  • Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg mL. Stock solutions should be ap
  • Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep