• Product NameCREB(Ab-133) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.
• Target NameCREB
• Alternative Names    CREB-1; CREB1;
• Accession No. Swiss-Prot: P16220
  NCBI Protein: NP _004370.1
• UniprotP16220
• Gene ID1385;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.

• Western blot analysis of extracts from 293, HT29 and Hela cells using CREB(Ab-133) Antibody #21052.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-133) Antibody #21052(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting