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Akt(Ab-473) Antibody #21054

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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
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  • Product NameAkt(Ab-473) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • ApplicationsWB IHC
  • Species ReactivityHu Ms
  • SpecificityThe antibody detects endogenous level of total Akt protein.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt.
  • Target NameAkt
  • Alternative Names    AKT; C-AKT; PKB; PKB-alpha; RAC
  • Uniprot P31749
  • Gene ID 207;
  • Accession No. Swiss-Prot: P31749
    NCBI Protein: NP_001014431.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Published Papers

Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-��B, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737, , (2020),
PMID: 32413737
Downregulation of GLYAT facilities tumor growth and metastasis and poor clinical outcomes through the PI3K/AKT/Snail pathway in human breast cancer., , (2020),
Study on the Therapeutic Effects of Traditional Chinese Medicine on Insulin Resistance in Obese Mice by Modulating Intestinal Function., , (2020),
Screening active components from Rubus amabilis for pancreatic ��-cells protection. In Pharm Biol on 2020 Dec by Min Sun, Tiantian Zhu, et al..PMID: 32659127, , (2020),
PMID: 32659127
View All Published Papers Glutathione S-transferases P1 protects breast cancer cell from adriamycIn-Induced cell death through promotIng autophagy. In Cell Death Differ.on 2019 Jan 25. by Dong X, Yang Y,et al.. PMID: 30683915, , (2019),
PMID: 30683915
RheIn from Rheum rhabarbarum Inhibits Hydrogen-Peroxide-Induced Oxidative Stress in IntestInal Epithelial Cells Partly through PI3K/Akt-Mediated Nrf2/HO-1 Pathways. In J Agric Food Chem on 2019 Mar 6, by Zhuang S, Yu R, et al..PMID: 30779558, , (2019),
PMID: 30779558
Synthesis of a novel platInum(II) complex with 6,7-dichloro-5,8-quInolInedione and the study of its antitumor mechanism In testicular semInoma. In J Inorg Biochem on2019 Aug by Li Z1, Zhou J1, et al.. PMID:31055215, , (2019),
PMID: 31055215
IcariIn reduces high glucose?Induced endothelial progenitor cell dysfunction via InhibitIng the p38/CREB pathway and activatIng the Akt/eNOS/NO pathway. In Exp Ther Med on 2019 Dec by Chen S, Wang Z, et al..PMID:31772646, , (2019),
PMID: 31772646
Ginkgo biloba Extract EGb761 Attenuates Hyperhomocysteinemia-induced AD Like Tau Hyperphosphorylation and Cognitive Impairment in Rats. In Curr Alzheimer Res.On 2018 by Zeng K, Li M et al..PMID: 28847282, , (2018),
PMID: 28847282
Inhibition of miRNA?21 attenuates the proliferation and metastasis of human osteosarcoma by upregulating PTEN.In Exp Ther Med.On 2018 Jan by Li C, Xu B et al..PMID:29434694, , (2018),
PMID: 29434694
Essential Role of Polo-like Kinase 1 (Plk1) Oncogene in Tumor Growth and Metastasis of Tamoxifen-Resistant Breast Cancer.In Mol Cancer Ther. On 2018 Apr by Jeong SB, Im JH et al..PMID:29437878, , (2018),
PMID: 29437878
Tetrahydroxystilbene Glucoside Regulates Proliferation, Differentiation, and OPG/RANKL/M-CSF Expression in MC3T3-E1 Cells via the PI3K/Akt Pathway.In Molecules. On 2018 Sep 10 by Fan YS, Li Q et al..PMID: 30201908, , (2018),
PMID: 30201908
Tetrahydroxystilbene Glucoside Regulates Proliferation, Differentiation, and OPG/RANKL/M-CSF Expression in MC3T3-E1 Cells via the PI3K/Akt Pathway.In Molecules.On 2018 Sep 10 by Fan YS, Li Q et al..PMID:30201908, , (2018),
PMID: 30201908
1α,25-Dihydroxyvitamin D3 inhibits aflatoxin B1-induced proliferation and dedifferentiation of hepatic progenitor cells by regulating PI3K/Akt and Hippo pathways.In J Pharm Pharmacol.On 2018 Jan Gong YH, Hua N et al..PMID:28994117 , , (2018),
PMID: 28994117
Silencing of advanced glycosylation and glycosylation and product-specific receptor (RAGE) inhibits the metastasis and growth of non-small cell lung cancer.In Am J Transl Res on 2017Jun 15 by Yan Xia Yu, Wen Chong Pan,et al..PMID: 28670367, , (2017),
PMID: 28670367
Efficient Expression of Stable Recombinant Human Insulin-Like Growth factor-1 Fusion With Human Serum Albumin in Chinese Hamster Ovary Cells.In Prep Biochem Biotechnol on 2017 Aug 9 by Aini Wan , Dongsheng Xu,et al..PMID: 28281882, , (2017),
PMID: 28281882
Hydrogen Sulfide Ameliorated L-NAME-induced Hypertensive Heart Disease by the Akt/eNOS/NO Pathway. In Exp Biol Med (Maywood) on 2017 Dec by Sheng Jin, Xu Teng,et al..PMID: 28971696, , (2017),
PMID: 28971696
Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity.In Biochem Biophys Res Commun on 2017 Jan 29 by Yang Ke, Tianhao Bao,,et al..PMID: 27998773, , (2017),
PMID: 27998773
MicroRNA-19a functions as an oncogene by regulating PTEN/AKT/pAKT pathway in myeloma.In Leuk Lymphoma on 2017 Apr by Xiaoying Zhang, Yafang Chen,et al..PMID: 27830963, , (2017),
PMID: 27830963
Radix Puerariae and Fructus Crataegi Mixture Inhibits Renal Injury in Type 2 Diabetes via Decreasing of AKT/PI3K.In BMC Complement Altern Med on 2017 Sep 8 by Zhengyue Chen, Yanyan Yuan ,et al..PMID: 28886733, , (2017),
PMID: 28886733
Phosphatase and Tensin Homolog Deleted on Chromosome 10 Degradation Induced by NEDD4 Promotes Acquired Erlotinib Resistance in Non-Small-Cell Lung Cancer.In Tumour Biol on 2017 Jul by Huake Sun, Huiwen Ma,et al..PMID: 28714370, , (2017),
PMID: 28714370
Tunneling Nanotubes Promote Intercellular Mitochondria Transfer Followed by Increased Invasiveness in Bladder Cancer Cells.In Oncotarget on 2017 Feb 28 by Jinjin Lu, Xiufen Zheng,et al..PMID: 28107184, , (2017),
PMID: 28107184
Exosomes Derived from Akt �\Modified Human Umbilical Cord Mesenchymal Stem Cells Improve Cardiac Regeneration and Promote Angiogenesis via Activating Platelet�\Derived Growth Factor D. In Stem Cells Transl Med on 2017 Jan by Jie Ma, Yuanyuan Zhao, et al..PMID:28170176, , (2017),
PMID: 28170176
Bruceine D induces apoptosis in human chronic myeloid leukemia K562 cells via mitochondrial pathway.In Am J Cancer Res on 2016 Mar 15 by Jian-Ye Zhang, Min-Ting Lin et al..PMID: 27186433, , (2016),
PMID: 27186433
Hepatic SATB1 induces paracrine activation of hepatic stellate cells and is upregulated by HBx.In Sci Rep.On 2016 Nov 24 by Gong J, Tu W et al..PMID:27883059, , (2016),
PMID: 27883059
miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway.In Int J Oncol.On 2016 May by J Han, F Zhao et al..PMID: 26936292, , (2016),
PMID: 26936292
Neuroprotective effects of ginseng protein on PI3K/Akt signaling pathway in the hippocampus of D-galactose/AlCl3 inducing rats model of Alzheimer’s disease.In J Ethnopharmacol on 2016 Feb 17 by Hongyan Li, Tingguo Kang et al..PMID: 26721223, , (2016),
PMID: 26721223
TDRG1 regulates chemosensitivity of seminoma TCam-2 cells to cisplatin via PI3K/Akt/mTOR signaling pathway and mitochondria-mediated apoptotic pathway.In Cancer Biol Ther on 2016 Jul 2 by Yu Gan , Yong Wang et al..PMID:27104982, , (2016),
PMID: 27104982
H2O2 treatment or serum deprivation induces autophagy and apoptosis in naked mole-rat skin fibroblasts by inhibiting the PI3K/Akt signaling pathway.In Oncotarget on 2016 Dec 20 by Shanmin Zhao, Li Li et al..PMID:27863375, , (2016),
PMID: 27863375
Diazoxide protects L6 skeletal myoblasts from H2O2-induced apoptosis via the phosphatidylinositol-3 kinase/Akt pathway.In Inflamm Res on 2016 Jan by Wei Chen , Yan Liu et al..PMID: 26525360 , , (2016),
PMID: 26525360
The Effects of Xiangqing Anodyne Spray on treating acute soft-Tissue injury mainly depend on suppressing activations of AKT and p38 pathways.In Evid Based Complement Alternat Med on 2016 by Shudong Wang , Tao Li et al..PMID:27190541, , (2016),
PMID: 27190541
An alternatively spliced variant of CXCR3 mediates the metastasis of CD133+ liver cancer cells induced by CXCL9.In Oncotarget on 2016 Mar 22 by Qiang Ding, Yujia Xia et al..PMID:26883105, , (2016),
PMID: 26883105
Genipin derivatives protect RGC-5 from sodium nitroprusside-induced nitrosative stress. In Int J Mol Sci on 2016 Jan 19 by Rikang Wang, Jiaqiang Zhao,et al..PMID:26797604 , , (2016),
PMID: 26797604
Effects of diesel exhaust particles on microRNA-21 in human bronchial epithelial cells and potential carcinogenic mechanisms.In Mol Med Rep on 2015 Aug by Fang Zhou, Suli Li et al..PMID:25901472 , , (2015),
PMID: 25901472
Short-Term Curcumin Gavage Sensitizes Insulin Signaling in Dexamethasone-Treated C57BL/6 Mice.In J Nutr.On 2015 Oct by Tian L, Zeng K et al..PMID:26338887, , (2015),
PMID: 26338887
Acetylcholine inhibits tumor necrosis factor α activated endoplasmic reticulum apoptotic pathway via EGFR‐PI3K signaling in cardiomyocytes.In J Cell Physiol on 2015 Apr by Yi Miao 1 , Xue-Yuan Bi et al..PMID:25201632, , (2015),
PMID: 25201632
Effect of Fructus Ligustri Lucidi on osteoblastic like cell-line MC3T3-E1.In J Ethnopharmacol on 2015 Jul 21 by Qin Li, Ying-sai Fan et al..PMID:25978950, , (2015),
PMID: 25978950
Combinational treatment of curcumin and quercetin against gastric cancer MGC-803 cells in vitro Molecules on 2015 Jun 22 by Jian-Ye Zhang , Min-Ting Lin et al..PMID:26111180 , , (2015),
PMID: 26111180
SNAILs promote G1 phase in selected cancer cells.In Int J Oncol on 2015 Nov by Ya-Lan Wu , Jian-Xin Xue et al ..PMID:26352801, , (2015),
PMID: 26352801
The use of hollow mesoporous silica nanospheres to encapsulate bortezomib and improve efficacy for non-small cell lung cancer therapy.In Biomaterials.On 2014 Jan by Shen J, Song G et al..PMID:24125776, , (2014),
PMID: 24125776
The effects of cannabidiol and its synergism with bortezomib in multiple myeloma cell lines. A role for transient receptor potential vanilloid type-2.In Int J Cancer.On 2014 Jun 1 by Morelli MB, Offidani M et al..PMID: 24293211, , (2014),
PMID: 24293211
The role of NAD+-dependent isocitrate dehydrogenase 3 subunit α in AFB1 induced liver lesion.In Toxicol Lett on 2014 Jan 30 by Chi Yang , Jue Fan et al..PMID:24211421, , (2014),
PMID: 24211421
Activation of liver X receptor enhances the proliferation and migration of endothelial progenitor cells and promotes vascular repair through PI3K/Akt/eNOS signaling pathway activation.In Vascul Pharmacol on 2014 Sep et al..PMID: 24892989, , (2014),
PMID: 24892989
Antitumour activity of the recombination polypeptide GST-NT21MP is mediated by inhibition of CXCR4 pathway in breast cancer.In Br J Cancer on 2014 Mar 4 by Tiefeng Jin, Jingchun Jin et al ..PMID:24629131, , (2014),
PMID: 24629131
FHL1C induces apoptosis in notch1-dependent T-ALL cells through an interaction with RBP-J. In BMC Cancer on 2014 Jun 22 by Wei Fu, Kai Wang, et al..PMID:24952875 , , (2014),
PMID: 24952875
The effects of cannabidiol and its synergism with bortezomib in multiple myeloma cell lines. A role for transient receptor potential vanilloid type-2. In Int J Cancer on 2014 Jun 1 by Maria Beatrice Morelli, Massimo Offidani, et al..PMID:24293211, , (2014),
PMID: 24293211
Ze-yang Ding, Guan-nan Jin, Hui-fang Liang Transforming growth factor ¦Â induces expression of connective tissue growth factor in hepatic progenitor cells through Smad independent signaling., Cellular Signalling., 25(10):1981¨C1992 (2013),
PMID: 23727026
Loss of Fatty Acid Synthase Inhibits the "HER2-PI3K/Akt Axis" Activity and Malignant Phenotype of Caco-2 Cells.In Lipids Health Dis on 2013 Jun 1 by Nan Li, Heng Lu,et al..PMID:23725225, , (2013),
PMID: 23725225
Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. In Toxicol Lett on 2013 Mar 27 by Hongxia Zhang, Junjie Hou, et al..PMID: 23353032, , (2013),
PMID: 23353032
Transforming growth factor β induces expression of connective tissue growth factor in hepatic progenitor cells through Smad independent signaling. In Cell Signal on 2013 Oct by Ze-yang Ding , Guan-nan Jin, et al..PMID: 23727026, , (2013),
PMID: 23727026
FLZ alleviates the memory deficits in transgenic mouse model of Alzheimer's disease via decreasing beta-amyloid production and tau hyperphosphorylation. In PLoS One on 2013 Nov 4 by Xiu-Qi Bao, Ning Li, et al..PMID: 24223757, , (2013),
PMID: 24223757
Nan Li, Xiaodong Bu, Peng Wu The ¡®¡®HER2¨CPI3K/Akt¨CFASN Axis¡¯¡¯ Regulated Malignant Phenotype of Colorectal Cancer Cells., Lipids, 47:403¨C411 (2012),
PMID: 22218925
Monocyte to macrophage differentiation-associated (MMD) positively regulates ERK and Akt activation and TNF-α and NO production in macrophages.In Mol Biol Rep on 2012 May by Liu Q, Zheng J, et al..PMID:22203480, , (2012),
PMID: 22203480
The “HER2–PI3K/Akt–FASN Axis” Regulated Malignant Phenotype of Colorectal Cancer Cells. In Lipids on 2012 Apr by Nan Li, Xiaodong Bu, et al..PMID:22218925, , (2012),
PMID: 22218925
Song Chen, Hedeel Guy Evans, David R FAM129B/MINERVA, a Novel Adherens Junction-associated Protein, Suppresses Apoptosis in HeLa Cells, J. Biol. Chem., 286(12):10201-10209 (2011),
PMID: 21148485
Ischemic Postconditioning Attenuates Liver Warm Ischemia-Reperfusion Injury Through Akt-eNOS-NO-HIF Pathway.In J Biomed Sci on 2011 Oct 28 by Jia Y Guo, Tong Yang,et al..PMID:22035453, , (2011),
PMID: 22035453
Rb1 postconditioning attenuates liver warm ischemia-reperfusion injury through ROS-NO-HIF pathway.In Life Sci on 2011 Mar 28 by Guo Y, Yang T,et al..PMID:21300075, , (2011),
PMID: 21300075
The effects of artesunate on the expression of EGFR and ABCG2 in A549 human lung cancer cells and a xenograft model.In Molecules on 2011 Dec 19 by Ma H1, Yao Q, et al..PMID:22183882, , (2011),
PMID: 22183882
Canonical notch pathway protects hepatocytes from ischemia/reperfusion injury in mice by repressing reactive oxygen species production through JAK2/STAT3 signaling.In Hepatology on 2011 Sep 2 by Yu HC, Qin HY,et al..PMID:21633967, , (2011),
PMID: 21633967
FAM129B/MINERVA, a novel adherens junction-associated protein, suppresses apoptosis in HeLa cells. In J Biol Chem on 2011 Mar 25 by Song Chen, Hedeel Guy Evans, et al..PMID: 21148485 , , (2011),
PMID: 21148485
Hyun Seung Ban, Masaharu Uno, Hiroyuki Nakamura Suppression of hypoxia-induced HIF-1 [alpha] accumulation by VEGFR inhibitors: Different profiles of AAL993 versus SU5416 and KRN633. , Cancer letters, 296(1):17-26 (2010),
PMID: 20378243
Massimo Nabissi, Maria Beatrice Morelli1, Consuelo Amantini TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner., Carcinogenesis, 31(5):794-803 (2010),
PMID: 20093382
Transactivation of human osteopontin promoter by human T-cell leukemia virus type 1-encoded Tax protein. In Leuk Res on 2010 Jun by Zhang J, Yamada O, et al..PMID:19767100, , (2010),
PMID: 19767100
Jing Zhang, Osamu Yamada, Yoshihisa Matsushita Transactivation of human osteopontin promoter by human T-cell leukemia virus type 1-encoded Tax protein., Leukemia Research, 34(6):763-768 (2009),
PMID: 19767100
A Proteomic approach for protein‐profiling the oncogenic ras induced transformation (H‐, K‐, and N‐Ras) in NIH/3T3 mouse embryonic fibroblasts. In Proteomics on 2008 Aug by Seyoon Kim, Yong Zu Lee, et al..PMID:18601226, , (2008),
PMID: 18601226
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WBWestern Blotting




FCFlow Cytometry



DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity




Dm Drosophila melanogaster

C Caenorhabditis elegans



B Bovine 

D Dog



ChHm Chinese Hamster