• Product NameVEGFR2(Ab-951) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total VEGFR2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.949~953 (K-D-Y-V-G) derived from Human VEGFR2.
• Target NameVEGFR2
• Alternative Names    FLK1; KDR; VGFR2; VGR2; kinase insert domain receptor
• Accession No. Swiss-Prot: P35968
  NCBI Protein: NP_002244.1
• UniprotP35968
• Gene ID3791;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 230kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions

Zeng H, et al. (2001) J Biol Chem. 276(35): 32714-32719.
Dougher M, et al. (1999) Oncogene. 18(8): 1619-1627.

• Western blot analysis of extracts from SKOV3 cells using VEGFR2(Ab-951) Antibody #21079 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Ab-951) Antibody #21079(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Ab-951) Antibody #21079.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting