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p53(Ab-15) Antibody

21085
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  Catalog No. Package Size Price
21085-1 50ul $189.00
21085-2 100ul $279.00

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Description
  • Product Name p53(Ab-15) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC IF IP
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total p53 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from Human p53.
  • Target Name p53
  • Alternative Names Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;
  • Accession No. Swiss-Prot: P04637
    NCBI Protein: NP_000537.3
  • Concentration 1.0mg/ml
  • Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 53kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from MDA and JK cells using p53(Ab-15) Antibody #21085.

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Ab-15) Antibody #21085(left) or the same antibody preincubated with blocking peptide(right).

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using p53(Ab-15) Antibody #21085.

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    Immunoprecipitation analysis of extracts of 293T cells incubated with p53 (Ab-15) Antibody

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    All lanes : p53(Ab-15) Antibody (21085) at 1/1000 dilutionLane 1 : Rat breast tissue;Lane 2 : Rat colon tissue;Lysates/proteins at 40 µg per lane.Predicted band size : 53 kDaObserved band size : 55 kDa

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    21085 at 1/150 staining rat colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4°C. A Goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    21085 at 1/150 staining rat breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4°C. A Goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    Immunofluorescent analysis of formalin-fixed, paraffin-embedded rat breast with 21085 at 1/100 dilution. A Goat Anti-Rabbit IgG

  • p53(Ab-15) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from mouse brain tissue using p53 (Ab-15) Antibody #21085.

Background

p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

    Please let us know if you have published research using #21085 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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