• Product NameCAPN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total CAPN1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 543-713 amino acids of Human Calpain-1 catalytic subunit
• Target NameCAPN1
• Alternative Names    Calpain-1 catalytic subunit, CANP, muCL, CANP1, CANPL1, muCANP
• Accession No. Swiss-Prot:P07384
  Gene ID:823;
• UniprotP07384
• Gene ID823;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 82kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:200-1:1000

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

• Gel: 10%SDS-PAGE
  Lysate: 30 µg 293T cell lysate
  Primary antibody: 1/450 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting