• Product NameHDAC2(Ab-394) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total HDAC2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 392~396 (E-D-S-G-D) derived from Human HDAC2.
• Target NameHDAC2
• Alternative Names    HD2; Histone deacetylase 2;
• Accession No. Swiss-Prot: Q92769
  NCBI Protein: NP_001518.2
• UniprotQ92769
• Gene ID3066;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes

Tsai SC, et al.(2002)J Biol Chem; 277(35): 31826-33

• Western blot analysis of extracts from 293 and Hela cells using HDAC2(Ab-394) Antibody #21140.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2(Ab-394) Antibody #21140(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using HDAC2(Ab-394) Antibody #21140.
• Western Blot analysis of Hela /293 cells using HDAC2 Polyclonal Antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting